Business And Society

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One doesn’t normally associate beautiful jewelry with the time of the Spanish Inquisition. But in the Smithsonian Institution’s collection of jewelry, there is an exquisite necklace holder stand and a necklace badge holder of diamonds and emeralds.

It is a spectacular double row of diamonds and emeralds finishing in a chandelier of emeralds. There’s unfortunately no information about the provenance of this necklace holders. The large diamonds and Columbian emeralds were most likely carved in India in the 17th century. This would make them one of the earliest examples of cut gemstones in the Smithsonian’s Collection. They are really only stories surrounding this necklace and what kind of wall necklace holder display stand were used in this era. They indicate that it was worn at times by Spanish and French royalty. In the early 20th century, it was bought by the Maharajah of Indore, whose son sold the necklace in 1947 to Harry Winston. Winston subsequently sold the necklace to Mrs. Cora Hubbard Williams of Pittsburgh. She bequeathed it to the Smithsonian in 1972.

Emeralds are a type of crystal known as beryls. Beryls are normally clear crystals, but when placed with chromium or vanadium, they keep various gradations of green. The shiniest green are the rarest emeralds and many people actually like an emerald that has a blue-green tint.

Prior to the 16th century, the only known emerald deposits were in Cleopatra’s Egyptian mines. But after emeralds were found in Columbia, those later became the “gold standard” in emeralds. Columbian emeralds have been discovered by archaeologists among artifacts of such tribes as the Inca, Maya, Aztec, Toltec and the almost unknown Chibcha Indians.

HPLC analysis is an interesting process utilizing stationary and solid phase with a mobile or liquid phase.  A glass or metal, or even plastic tube, called a column, is used to house the stationary phase, usually silica beads or some sort of porous solid laminated with targeted compounds at varying levels so it attracts those items which need separating.  As the reactive solvent including the compound moves through the column, separate molecules or even atoms can attach themselves  to the stationary phase, thus separating the compound into its constituents.

We have always used so many techniques in the lab over the years but one of these included the synthesis of individual proteins.

This all commences with the purifying of amino acids and this can be fulfilled by using HPLC columns to separate the separate amino acids from different proteins.  The column has a stationary phase that can separate the individual amino acids as the proteins make their way through the column.  The proteins go through a liquification and homogenization process via a series of solvents, and the solvent becomes a carrier, known as the mobile phase.  The proteins are separated in an efficienct way as it is pumped through the system.

Particle size as well as pore size in the stationary phase of an HPLC column is critical to the velocity at which the phase can separate the compound travelling through it.  Silica is a popular substance used in reverse-phase chromatography, and this can be applied in a HPLC chromatography system.  Silica is not the only substance, and great care should be taken when using certain acidic solvents.  Temperature is also important, as excessive heat can damage the silica.  The electrical make up, as well as pore size found on the beads are the main benefits of silica.